Moreover, to validate the effects of IL27 on β-catenin signaling, we analyzed the relationship of IL27 with the main upstream and target molecules of the catenin signaling pathway, including (LRP6, DVL1, PSEN1, CTNNB1, GSK3B, APC, APC2, AXIN1, AXIN2, TCF12, MYC, FOSL1 and VEGFA) (44, 45), using RNA-seq data from the TCGA cohort of patients with melanoma. The gene discussed is GSK3B; the disease is melanoma.