LSR and colon carcinoma: As proof-of-concept, we generated a knockout of the gene encoding the protein LSR (lipolysis-stimulated lipoprotein receptor) in the human colon carcinoma cell line HCT116, since we previously succeeded in knocking out the lsr gene with the conventional CRISPR/Cas9 mutagenesis approach in this cell line (Hemmasi et al., 2015).