HLA-A and melanoma: To demonstrate functionally relevant recognition of our prediction of PHOX2B presentation on HLA-A*23:01, we pulsed the HLA-A*23:01 PHOX2B− melanoma cell line WM873 with the QYNPIRTTF peptide, showing induction of antigen-specific killing in peptide-pulsed cells and no cytotoxicity in cells pulsed with mismatched peptide (Extended Data Fig. 14d).