To select those peptides with the highest potential as putative immunotherapeutic targets, we prioritized peptides on the basis of pMHC binding affinity, HLA allele frequency, degree of differential expression of parent genes, relative abundance on MHC as compared to other peptides, recurrence across multiple neuroblastoma tumours and relevance to neuroblastoma biology based on the published literature23–25. Here, HLA-C is linked to neuroblastoma.