Obesity may alter the functioning of pancreatic β-cells and exacerbates insulin resistance (which is already increased as a result of pregnancy).22 33 36 38 42 43 Smoking has also been associated with insulin resistance, via processes including hormonal secretions (eg, growth hormone) that counteract insulin action.44 45 Thus, although there was no direct measure of insulin resistance in this study, it is possible that women who were obese or smoked during pregnancy had a higher degree of insulin resistance. The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.