Then the immunohistochemistry (IHC) staining which represented the protein expression of GSDMs were obtained through the Human Protein Atlas database (THPA) (Supplementary Figure 1), showing that the protein expressions of GSDMB, GSDMD, and GSDME were higher in HCC tissue compared with normal tissue, which is in accordance with the tendency of their mRNA expression. The gene discussed is GSDMD; the disease is hepatocellular carcinoma.