qRT-PCR results showed that the RNA level of MMP14 decreased after MELTF-AS1 was silenced in osteosarcoma cells, and the decreased MMP14 returned to normal level when silencing MELTF-AS1 was combined with transfection of miR-485-5p inhibitors (Figure 5B). The gene discussed is MELTF; the disease is osteosarcoma.