These criteria use the results of several investigations to aid diagnosis, including Aspergillus skin tests, sputum cultures, peripheral eosinophilia, total serum immunoglobulin E (total IgE), A. fumigates-specific IgE, A. fumigates-specific immunoglobulin G (IgG), and radiological findings.15 Various cut-off values for these investigations have been proposed as several of these investigations may be abnormal in other fungal or related diseases, and there can be difficulties with the effects of treatment on some parameters.15 This evidence concerns the gene IGHE and Increased total eosinophil count.