The luciferase activities were significantly decreased in ESCC cell lines, KYSE-70 and KYSE-180 co-transfected with the pEZX-MT04 vectors containing miR-375 plus pEZX-MT05 vector containing ERBB2 3′UTR wild type sequence, compared to that co-transfection of either miR-375 plus 3′UTR mutant sequence or scrambled control plus ERBB2 3′UTR wild type one. Here, ERBB2 is linked to esophageal squamous cell carcinoma.