Furthermore, the results from Western blot analysis also confirmed that the expressions of β-catenin as well as the key downstream genes (Cyclin D1, c-Myc, and MMP-7) in the Wnt/β-catenin signaling pathway were significantly affected by knockdown or overexpression of MUC1 in two ICC cell lines 25-27,42. The gene discussed is MMP7; the disease is intrahepatic cholangiocarcinoma.