Therefore, we further analyzed the expression of MUC1 in another 12 ICC samples with lymphatic metastasis (n = 6) or without lymphatic metastasis (n = 6) and found that the mRNA and protein levels of MUC1 were significantly higher in ICC cases with lymphatic metastasis compared to those without lymphatic metastasis (protein levels P value = 0.003; mRNA levels P value = 0.002; Figure 1D). This evidence concerns the gene MUC1 and Lymphatic Metastasis.