The study revealed that: (1) VISTA gene expression was upregulated in high versus low glioma grades, ((2) VISTA protein exhibited elevated expression levels in high-grade glioma, (3) VISTA expression positively correlated with other critical immune checkpoint regulators, (4) genes, which are known to be linked to an anti-tumor signature, were inhibited in the glioma microenvironment, (5) elevated VISTA transcript levels negatively correlated with the IDH mutation in patients, and (6) high VISTA transcript levels associated to weak overall patient survival. Here, VSIR is linked to central nervous system cancer.