However, WT-TRMT6/TRMT61A but not TRMT6R377L/TRMT61AD181A mutant (Mut) overexpression in TRMT6/TRMT61A depleted CSCs could restore signals of m1A (Fig. 2c, d), indicating that TRMT6/TRMT61A directly regulate RNA m1A modification in HCC CSCs. The gene discussed is TRMT61A; the disease is hepatocellular carcinoma.