Further detection by IHC showed that the expressions of AT1R, PLC-β1 and CaM in HCC tissues were significantly higher than those in para-carcinoma tissues, and with the increase of Edmondson-Steiner pathological grade, the levels of the above-mentioned target proteins in HCC specimens increased to different degrees (Fig. 3D). The gene discussed is PLCB1; the disease is hepatocellular carcinoma.