To clarify whether the miR-485-5p-E2F1 axis participates in glioblastoma with CDDP treatment, the CCK8 assay was performed; it was observed that E2F1-OE upregulated cell viability, while this effect was reversed by CDDP and miR-485-5p mimic and aggravated by inhibitor (Figure 4a). The gene discussed is E2F1; the disease is glioblastoma.