To demonstrate the potential of prepared core/shell cMIP NPs in biomedical applications, we prepared single QD‐cored cMIP (QD@cMIP) NPs against two typical cancer biomarkers including human epidermal growth factor receptor‐2 (HER2) and transmembrane glycoprotein non‐metastatic gene B (GPNMB) and achieved differentiation of triple‐negative breast cancer (TNBC) cells over other cell lines via fluorescence imaging. This evidence concerns the gene CMIP and cancer.