In this study, we firstly confirmed that ANXA2 protein in exosomes derived from ovarian cancer varies with the content of ANXA2 in the host cells, and the expression of ANXA2 was upregulated in HMrSV5 cells after co‐cultured with OVCAR3‐exo or ES‐2‐exo, indicating that ANXA2 can not only regulate the formation and release of exosomes secreted by ovarian cancer cells, but also transfer to HMrSV5 cells via exosomes, thus having potential effect on the biological characteristics of target cells. The gene discussed is ANXA2; the disease is ovarian carcinoma.