Figure 2(c) indicates that the highest increase in the mRNA levels of Runx1 occurred at week 1 after MI surgery compared to the levels in sham rats. In the Runt-related family (Runx1, Runx2, and Runx3), Runx1 expression was most significantly elevated (Figure 2(d)) according to the RNA-sequence analysis. We confirmed these results using western blotting and found that Runx1 was upregulated in the infarct area 1 week after MI surgery (Figures 2(e) and 2(f)). Here, RUNX2 is linked to myocardial infarction.