By overexpressing JAGGED1 in cells transfected with shRNA-SOX12, when compared with empty plasmid transfected cells, JAGGED1 overexpression partially rescued the spheroidizing ability of osteosarcoma cells transfected with shRNA-SOX12 (Figures 8(a) and 8(b)). The gene discussed is JAG1; the disease is osteosarcoma.