After that, we conducted tumor stemness-related assays, and we found that the osteosarcoma cells overexpressing SOX12 are more capable of forming spheres than the control (Figures 5(b) and 5(c)), and the stemness markers of the 143B cells overexpressing SOX12 are higher than that of the control group (Figure 5(d)). Here, SOX12 is linked to osteosarcoma.