From our in vivo stemness assay, where we injected 1 × 105 143B osteosarcoma cells mixed with 100 μl DMEM into nude mice, we observed that after 40 days, only one of the knockdown SOX12 group had successfully grown a tumor, while all in the NC group had a tumor (Figures 4(e)–4(g)). This evidence concerns the gene SOX12 and neoplasm.