SOX12 and neoplasm: After osteosarcoma cells were transfected with shRNA-SOX12 lentivirus, their ability to form spheroids was decreased, which was manifested in that the osteosarcoma cells that knock down SOX12 form less spheroids; the number of balls in the spheroidization experiment represents the stemness of tumor cells; this means that knocking down SOX12 can inhibit the stemness of osteosarcoma stem cells (Figures 4(a) and 4(c)).