As shown in Fig. 6A–C, the expression levels of the upstream molecules and core kinase cassette proteins, such as Kibra (WWC1), Merlin/NF2, pLATS1 (Ser909)/pLATS2 (Ser380), pMST1/2 (Thr183) as well as pYAP (Ser127) were upregulated or downregulated at protein levels accompanied by overexpressing or knocking down ARSD in MDA-MB-231 or MCF-7 BC cells, respectively. This evidence concerns the gene WWC1 and breast cancer.