In our study, G12406A (Val24Ile in ND5, defining mtDNA sub-haplogroup F1) and T16298C/C16304T/T16362C (D-loop, defining mtDNA sub-haplogroup F3) were detected, and both had significantly lower frequencies in COVID-19 patients (P = 0.043 and 0.018, respectively). The gene discussed is MT-ND5; the disease is COVID-19.