This study aimed to explore the roles of miR-889-3p and HIPK1 in LC cells and the regulatory mechanism of miR-889-3p/HIPK1 axis in LC cells, thus proposing the hypothesis that miR-889-3p refrained LC cell proliferation and EMT via down-regulating HIPK1. This evidence concerns the gene HIPK1 and laryngotracheoesophageal cleft.