Here, using deletion mutagenesis and amino acid substitution approaches in our established ischemia model, we systematically explored the impact of changes in Drp1 SUMOylation on the Drp1-Bcl-xL interaction, and the roles of the deSUMOylating enzyme SENP3 and Mff in regulating the Drp1-Bcl-xL interaction. This evidence concerns the gene SENP3 and ischemia.