We, for the first time, demonstrate that ferroptosis mediates the regulation of NSCLC development via targeting the miR-27a-3p/SLC7A11 pathway, revealing that, therefore, miR-27a-3p/SLC7A11 was an encouraging treatment biomarker and target for patients with NSCLC. The gene discussed is SLC7A11; the disease is non-small cell lung carcinoma.