To understand if this holds true also for MCL cells, we treated three different MCL cell lines with D4476 40μM or DMSO for 48h and analyzed the NF-κB signaling pathway, in particular the activating phosphorylation of CARD11 on Ser 652, of NF-κB p65 on Ser 536, of the upstream IKKα/β, and of IkBα on Ser 32. The gene discussed is CHUK; the disease is mantle cell lymphoma.