RUNX3 and leukemia: To evaluate the effects of Runx3 reduction, we first sorted green fluorescent protein (GFP)+ leukemia cells from Vector and Runx3 KD AML mice and confirmed that shRNA specific for Runx3 led to decreased RUNX3 expression by qRT-PCR and Western blot analysis (80.3% reduction of Runx3 mRNA expression; Figures 2B, , C and Supplementary Figure S2).