Transcriptional analysis showed that Runx3 knockdown decreased the expression level of cell cycle-related genes that RUNX3 binds to, such as Cdk4, Ccnd1, Ccnd2, Cdk2, Ccna1, and Ccnb1 (36.4%, 21.1%, 52.3%, 52.0%, 29.9%, and 45.4% reduction, respectively) in murine AML cells (Supplementary Figure S5). The gene discussed is CDK4; the disease is acute myeloid leukemia.