The immunohistochemistry (IHC) results showed that KHSRP, SRSF3 and RBM9 were located in nucleus, and the expression of SRSF3 and RBM9 were significantly lower in cancer than normal thyroid, while there was no significantly different between KHSRP, NOVA2 and PTBP2 in carcinoma and normal tissues (Figure 7F). The gene discussed is RBFOX2; the disease is carcinoma.