Western Blot analysis at a 540 nm wavelength for the plasma levels of adiponectin, leptin, resistin, IL-6,  IL-8, TNF-α, and CRP, in fasting state. Liver biopsies to assess NAFLD and categorized as steatosis, fibrosis, and steatohepatitis. This evidence concerns the gene IL6 and metabolic dysfunction-associated steatotic liver disease.