To investigate the relationship between SPP1 expressions with enzalutamide resistance in vivo, we used the bone metastatic CRPC model developed by Prof. Qian lab, Queen's Medical Research Institute, University of Edinburgh, following intracardiac inoculation of an androgen-dependent murine prostate cancer cell line cells in FVB/N male mice. The gene discussed is SPP1; the disease is prostate carcinoma.