Segmented filamentous bacteria (SFB), Enterococcus and Lactobacillus, which belong to the Firmicutes phylum, were not altered in Gsdmd-deficient mice; however, other members of Firmicutes, such as Clostridium cluster IV, Clostridium cluster XIVa, Eubacterium rectale (EREC), and Bacillus, which are potential beneficial microbiota for colitis (9), were obviously reduced in Gsdmd-/- mice. This evidence concerns the gene GSDMD and colitis.