Although the mRNA or protein level of full length GSDMD (GSDMD-FL) was not significantly regulated in the DSS-induced colitis model (Figures 1D, E), GSDMD was strongly cleaved to activated GSDMD-N form (p30 fragment) on Day 3 and the activation decreased on Day 6 during DSS-induced colitis (Figure 1E). The gene discussed is GSDMD; the disease is colitis.