Immunoblot analysis showed that the expression of cleaved Caspase 3 was similar in both whole colon and IECs between Adv-EV- and Adv-GSDMD-C treated mice, suggesting that Adv-GSDMD-C suppressed IEC pyroptosis during DSS-induced colitis (Supplementary Figure 5C). The gene discussed is CASP3; the disease is colitis.