Our study was based on standardized approaches not previously applied to CF MRSA isolates, that is, the BioFilm Ring test® (Chavant et al., 2007; Ren et al., 2007) and the microfluidics system BioFluxTM 200 (Benoit et al., 2010) which were adapted for use with the artificial sputum medium (ASM), a mucin-containing synthetic growth medium (Sriramulu et al., 2005; Dinesh, 2010) to test MRSA biofilm formation, and antibiotic efficacy against biofilm formation in conditions more relevant to the CF lung. The gene discussed is MUC5AC; the disease is cystic fibrosis.