Both the Western blot and IF staining experiments showed that LPS significantly increased the expression of cGAS and STING and promoted the phosphorylation of TBK, IRF3, P65, and IκBα in lung tissues of ALI mice, suggesting that LPS could activate STING-mediated IRF3/NF-κB signaling pathway (Figures 6A–F). Here, IRF3 is linked to acute respiratory distress syndrome.