The pathways, such as PI3K-Akt signaling pathway (hsa04151), complement and coagulation cascades (hsa04610), NF-κB signaling pathway (ko04065), cytokine-cytokine receptor interaction (ko04060), mitophagy-animal (ko04137) and TGF-β signaling pathway (ko04350), were related to ALI caused by LPS (Figure 2B). The gene discussed is TGFB1; the disease is acute respiratory distress syndrome.