Heterozygous rare TTN variants of unknown significance (VUS) were common (Supplementary Table 1), but in light of their high prevalence in other LGMD patients (Fichna et al., 2018), as well as in general population (Akinrinade et al., 2015), their causative role is much more disputable than that of CAPN3 variants found. This evidence concerns the gene CAPN3 and limb-girdle muscular dystrophy.