To investigate the function of FTX in RB, the pc-FTX (the overexpressing vector) and sh-FTX were transfected into Y79 and WER1-RB1 cells, and qRT-PCR results showed that the expression levels of FTX were increased in pc-FTX transfected cells and reduced in sh-FTX transfected cells compared with that in the control group (all p < 0.001, Figure 2(a)). This evidence concerns the gene FTX and retinoblastoma.