ROs have also been used to identify the disease mechanisms of Leber’s congenital amaurosis (LCA)-related RPE65 (Li et al. 2019), CEP290 (Shimada et al. 2017), AIPL1 (Lukovic et al. 2020) and CRX (Kruczek et al. 2021). This evidence concerns the gene CRX and Leber congenital amaurosis.