ROs have also been used to identify the disease mechanisms of Leber’s congenital amaurosis (LCA)-related RPE65 (Li et al. 2019), CEP290 (Shimada et al. 2017), AIPL1 (Lukovic et al. 2020) and CRX (Kruczek et al. 2021). The gene discussed is CEP290; the disease is Leber congenital amaurosis.