To further evaluate the role of the FRGS, GSEA was performed in the TCGA CRC cohort, and the results showed that the CAF-induced microenvironment was significantly related to the inflammatory response, TNF-α, IFN-α, IFN-γ, IL-6 and IL-2 (Fig. 4B), which are related to the immune response network. This evidence concerns the gene IL2 and colorectal carcinoma.