nucleatum-positive ESCCs harboring TP53 mutations had concomitant mutations in COL22A1. The frequency of F. nucleatum-negative ESCC harboring RBMXL3 and HDGFRP2 mutations was 57.14 and 42.85%, respectively, which are different from the values for the F. nucleatum-positive group. The gene discussed is RBMXL3; the disease is esophageal squamous cell carcinoma.