nucleatum-positive ESCC tumor tissues, and we found genes that were present only in F. nucleatum-positive tumor tissues, such as COL22A1, TRBV10–1, CSMD3, SCN7A and PSG11. This finding is different from previous reports showing that KRAS and BRAF frequently occur in CRCs [28]. This evidence concerns the gene CSMD3 and esophageal squamous cell carcinoma.