Overall, our bioinformatic reanalysis suggests that 85% of the now 52 RARS2 variants likely impact on splicing or expression of the gene and that most cases with RARS2 encephalopathy have at least one variant with this effect (46/54 with two variants, 7/54 with one variant) (Table S1). This evidence concerns the gene RARS2 and Encephalopathy.