Interestingly, in the PI3K signaling investigated in our study, we observed the downregulation of the BCL2-associated agonist of cell death encoded by the BAD gene along with the deregulation of EGF, FGFR2, FLT3LG, HRAS, IGF1R, PDGFA, PDGFRB, PIK3R3, SOS1, and TGFA. On the other hand, GLIS3 belongs to the GLIS protein family of zinc finger and occurs in many diseases, including neonatal diabetes mellitus, aplasia, hypoplasia, hypothyroidism, growth retardation, atrial septal defects, autoimmune diseases, and neurological disorders. This evidence concerns the gene PIK3R3 and atrial septal defect.