Heat-denatured recombinant FAR-1 or FAR-2 was co-injected at the highest dose tested (250 ng); no difference was observed when compared to S. pneumoniae-only injected flies, confirming that the addition of folded, recombinant FAR in a one-time dose alters the flies’ ability to deal with pathogenic infection (S4 Fig). The gene discussed is FAR1; the disease is infection.