Our bioinformatics prediction results showed differentially expressed mRNAs in NSCLC and adjacent tissues, including CTCFL, KRT5, LY6D, TMEM179, GBP6, TMEM, etc. qRT-PCR and WB were performed to reveal the expression of these mRNAs in BEAS2B and A549 cells. The gene discussed is KRT5; the disease is non-small cell lung carcinoma.