The analysis identified 12 reporter TFs of genes in cohort1_215_M4, of which 9 were associated with upregulated genes in NAFLD, including, ATF4, DDIT3, HDAC3, HNF4A, KLF5, NFYC, SREBF1, SREBF2, and YY1. 2 reporter TFs (VDR and WT1) are associated explicitly with downregulated genes in cohort_215_M4 between patients with NAFL and control samples. Here, WT1 is linked to non-alcoholic fatty liver.