Transcription regulatory networks analysis indicated that SREBF1, SREBF2, HNF4A, YY1, and KLF13 are the most prominent regulators of gene expression in disease-associated modules, 3 of which (SREBF1, SREBF2, and KLF13) were validated using the transcriptomics data generated from a mouse NAFLD model. This evidence concerns the gene KLF13 and metabolic dysfunction-associated steatotic liver disease.