In the presence of EX527 (500 mg/kg), a specific SIRT1 antagonist, CR-mediated protection on CIN was lost, proved by the higher renal HE scores, serum Cr, and BUN in the CM+EX527+CR than the CM+CR group (Figures 3(b)–3(d)). This evidence concerns the gene SIRT1 and cervical squamous intraepithelial neoplasia.