This study found that acetylation occurred at the pathogenic Escherichia Coli infection-related molecules, including acetylation at residues K449 (only acetylated in controls) in nucleolin (ID: P19338), K440 (only acetylated in controls) in tubulin alpha-1C chain (ID: F5H5D3), K61 (ratio of T/N=0.22, p=2.59E-04) and K326 (only acetylated in controls) in actin cytoplasmic 1 (ID: P60709), K394 (ratio of T/N=0.29, p =2.08E-02) and K336 (ratio of T/N=0.21, p=1.77E-04) in tubulin alpha-1B chain (ID: P68363), and K35 (only acetylated in controls) in ezrin (ID: P15311). The gene discussed is NUCLEOLIN; the disease is escherichia coli infection.