Notably, iron homeostasis pathway genes with altered expressions in pancreatic cancer tissues included FXN and MYC, while ferroptosis pathway genes with altered expressions included ALOX15, CBS, FDFT1, LPCAT3, RPL5, TP53, and TTC35. We found that iron metabolism genes, low expression of FXN, and high expression of MYC were associated with increasing risks of pancreatic cancer. This evidence concerns the gene RPL5 and familial pancreatic carcinoma.