Of note, given that ITGB8 expression was reported to be increased on activated human Tregs18, we also removed the gene signature of activated Tregs in the ITGB8 Treg signature and obtained similar survival prognostics as with the ITGB8 Treg total gene signature for all the tumor types we analyzed (Supplementary Fig. 5). Here, ITGB8 is linked to neoplasm.