NGEF and neoplasm: Reconstitution of WT Ephexin1 in Ephexin1-depleted H1299 cells was sufficient to increase anchorage-independent growth (Fig. 5E–G) and xenograft tumor in nude mice growth (Fig. 5H–J), but a more dramatic increase was seen in the presence of either the S16D/S18D or S16E/S18E mutant.