In the present study, we provide evidence that p32 promotes various carcinogenic properties of murine melanoma cell, and the expression of oncogenic transcription factors Snail, Slug, and Twist together with various key mesenchymal markers (N-cadherin, fibronectin, MMP2, and MMP9) and oncogenes (c-Myc and cyclin D1) and further validation on human melanoma cells (A375) signifies the involvement of p32 in melanoma progression, and metastasis. This evidence concerns the gene TWIST1 and melanoma.