To find out the possible role of p32 in melanoma tumorigenesis in vitro, we silenced p32 in murine melanoma B16F10 cells and human melanoma A375 cells using specific p32 shRNA and further our immunoblot and transcript level analysis results showed profound inhibition of p32 in both the murine (Fig. 1A) and human melanoma cells (Fig. 1B). The gene discussed is C1QBP; the disease is melanoma.