Next, to study the role of p32 in melanoma cells lung metastasis, we injected control and p32 shRNA-transfected B16F10 cells intravenously through the tail vein in C57BL/6 mice, and our results showed that the p32 shRNA-transfected B16F10 cells developed a very less numbers as well as small tumor foci in lungs (Fig. 5A) and their H&E staining of lung tissue sections showing the tumors formation in these tissues (Fig. 5B). This evidence concerns the gene C1QBP and melanoma.