Using flow cytometry analysis, we further demonstrated that the binding of biotin-conjugated TGFβ−1 to M1-like macrophages, but not to M0 and M2-like macrophages, was significantly increased after co-culturing with PDA cells compared to singly-cultured macrophages (Supplementary Fig. 1e). This evidence concerns the gene TGFB1 and Patent ductus arteriosus.