PTEN and neoplasm: A homozygous TP53 missense (c.517G>C, p.V173L) SNV, a homozygous PTEN frameshift (c.676_697delTCCTCCAATTCAGGACCCACAC, p.S226fs), and a homozygous RB1 deletion occurred in the founding subclone, indicating that these mutations were major primary driver mutations in this patient’s tumor.