2001; Patel and Rattan 2007; Kim et al. 2008; Wu et al. 2018). When compared with the expression levels in the NC group, ROCK-1 mRNA and protein expressions and MLCK protein expression were much increased in both antrum and jejunum in diarrhoea rats (Figure 7) but much decreased in Constipation rats (Figure 8), which were consistent with the abnormal regulatory responses to diarrhoea and constipation symptoms. This evidence concerns the gene ROCK1 and Constipation.